Using the ORIGIO Gradient Series and Sperm Wash, with the design elements described above, may help preserve sperm integrity and promote motility. All this would be for naught, however, if handling and preparation procedures employed by the laboratory are not optimized and well-controlled.
The general advice is to limit the time between sample production and preparation, ideally beginning as soon as the sample has liquefied. Where possible, it is also preferable to keep the time between preparation and use to a minimum though scheduling of procedures can make this tricky.
In all cases, however, the sample should be processed and kept before use at room temperature and not 37°C as the higher temperature may result in a faster rate of spontaneous DNA damage (especially in a CO2-enriched environment).1 It should be noted that the ORIGIO Series is designed to be used at room temperature.