It’s Not Over Yet: Upcoming Features
Dr Blazek concluded by announcing that his team has been at it again. They are currently finishing another update that will utilize PGTai 2.0’s SNP data to identify a genetic match between the embryo sample and the submitted parental samples, i.e. a parental quality check (parental QC).
To design this newest analysis, Dr Blazek and his team utilized a training set of 132 trios from 27 PGT-M families, including 14 consanguineous families. The trios were analyzed on the PGTai 2.0 SNP platform in numerous permutations with incorrect maternal DNA, paternal DNA, or both.
They then validated this algorithm with an independent test set of trios. The independent validation identified a 99.7% accuracy rate with 100% sensitivity and 99.4% specificity (Figure 5). Dr Blazek anticipated that this technology will impart peace of mind for clinicians and patients.
CooperSurgical’s Chain of Quality
Dr Gordon rounded out the session by speaking briefly about CooperSurgical’s ‘chain of quality’, which included RI WitnessTM, PGTai, and PGTai 2.0. He explained that RI Witness is a robust laboratory electronic witnessing system that prevents mismatch errors, streamlines workflows, and comprehensively documents laboratory actions.
Each year, an increasing number of PGT cycles are performed all over the world, which places a greater and more complex burden on the laboratory workload. Therefore, the accommodation of these additional steps in laboratory security protocols is imperative. Using RI Witness while biopsying, vitrifying, or prepping for transfer helps safeguard against sample misidentification.
PGTai is one of the most accurate platforms for identifying euploid embryos without human subjectivity and transcription errors. It is also the most sensitive platform for mosaic calling. PGTai 2.0 identifies haploidy and triploidy and provides 2PN verification. It can also identify the parental origin of aneuploidy and, soon, parental QC. Some of these features require parental cheek brush swabs and can be assessed both prospectively and retrospectively for all samples run on the PGTai 2.0 platform.