You will need the pre-warmed media, dishes, dispensing pipettor with tips, a pipetting device, a marker pen, timer and cryodevices.

Note that all steps are preformed at room temperature.
First, prepare the dishes.

Dispense 500uL Equilibration Solution (ES) into the well/dish.
Dispense 250uL Vitrification Solution (VS) to well 2 and well 3.
Transfer the specimen(s) to the ES and equilibrate for 5-15 minutes; bear in mind that the time depends on the embryo stage.

Note: Optimal timings must be confirmed in individual laboratory conditions.

Note all steps are performed at room temperature.

During equilibration, prepare the cryodevice taking care to maintain sterility.
This includes labelling with appropriate witnessing.

At the end of the equilibration time, transfer the specimen(s) to the well/dish containing VS1 and leave for 30 seconds.

Transfer the specimen(s) to the well/dish containing VS2 and leave for another 30 seconds.

At the end of the minute, load the embryo(s) onto the cryodevice in minimal volume. Remove any excess medium.

Cap the device.

Transfer to the liquid nitrogen and stir gently.

Cap the device, taking care to keep the sample under the liquid nitrogen.