What are the tests?
Atomic Fingerprinting employs a combination of the following:
Fourier Transform Infrared Spectroscopy (FTIR)
FTIR is a robust and precise tool used for the identification and verification of chemical samples. It measures and analyzes the absorption of infrared (IR) radiation of the sample at different wavelengths4. Different chemical functional groups will produce IR spectra in specific and reproducible patterns when analyzed by FTIR. The resulting FTIR spectra can be interpreted, with additional comparisons to known chemical libraries, to provide a positive identification of each sample component and a measurement of the amount. The spectral features from the FTIR analysis should be consistent with a mixture of fully saturated hydrocarbons.
Nuclear Magnetic Resonance Spectroscopy (NMR)
NMR is a sensitive and effective method to unambiguously and quantitatively characterize a material based on the quantum mechanical magnetic property of the atoms’ nuclei. It can detect minute differences in the unique local chemical, electronic and spatial environment of an atom, allowing for the identification of particular isotopes of the chemical elements present in the sample5. This means that a carbon in a straight-chained, fully saturated alkane will register a different spectrum from a branched, unsaturated, cyclic or aromatic carbon nucleus. The result of the analysis is based on the NMR spectrum of the material, and it is detecting chemical shifts attributable to double bonds, aromatic groups or other functional groups.
The FTIR and NMR analyses confirm that our oils only contain saturated C-C bonds.
Gas Chromatography with Flame Ionization Detection (GC-FID)
This technique relies upon chromatographic separation of sample components, by virtue of their relative volatility, followed by ionization and further separation according to their molecular weight (MW). Each component is identified through matching with an extensive computer database. Hence, GC-FID can detect any trace contaminants or molecular outliers, and illustrates the MW distribution patterns of alkanes within the mineral oil.
This analysis confirms there are no volatile components with boiling point <= 250⁰C.
As viscosity is an important physical property of mineral oil, we verify our oils using the kinematic viscosity method. Kinematic viscosity measurements differ from those of standard absolute viscosity measurements by taking into consideration the specific gravity of the sample. The method also measures the viscosity of the mineral oil at 40°C, which more closely emulates the temperature at which an embryologist would use equilibrated mineral oil for embryo culture or micromanipulation.
This analysis helps us ensure our oils have the right viscosity for handling.
Our ORIGIO Liquid Paraffin and SAGE Oil for Tissue Culture are both tested using Atomic Fingerprinting