Other Micromanipulation Micropipettes

Assisted hatching micropipettes are used to direct acidic solution (acidified Tyrodes) onto the zona pellucida to thin the zona or create a hole to enable the embryo to hatch.

Zona dissection micropipettes are used to mechanically cut an opening in the zona pellucida of an oocyte or embryo to enable assisted hatching and biopsy.

Micromanipulation pipettes for a variety of other uses in research are also available from our extensive range. Please contact your local representative for more information.

Availability

These products are available for sale in selected countries worldwide.

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What needles do you suggest I try for applying acid?

If you choose not to use laser-drilling of the zona pellucida, we recommend using the Assisted Hatching pipet (MAH-SM-(angle)) in combination with the Acidified Tyrodes media to create a hole in the zona. This means on the one side of the micromanipulator you need a double pipet holder to have the Assisted Hatching and the biopsy pipet both “in play.”

*Please enquire about special sizes and products for research use.

ORIGIO Assisted Hatching Micropipets

Small Hatching Micropipets (10 per box)

Reference Pipette Type Finish Style Tip Angle Internal Diameter (microns) Outer Diameter (microns) Distance tip to bend (mm)
MAH-SM-0 Straight Hatching  –  0  8-10µm  – 0.5mm
MAH-SM-20 Hatching  –  20  8-10µm  – 0.5mm
MAH-SM-25 Hatching  –  25  8-10µm  – 0.5mm
MAH-SM-30 Hatching  –  30  8-10µm  – 0.5mm
MAH-SM-35 Hatching  –  35  8-10µm  – 0.5mm

Large Hatching Micropipets (10 per box)

Reference Pipette Type Finish Style Tip Angle Internal Diameter (microns) Outer Diameter (microns) Distance tip to bend (mm)
MAH-LG-20 20º angled Hatching  –  20  10-12µm  – 0.5mm
MAH-LG-25 25º angled Hatching  –  25  10-12µm  – 0.5mm
MAH-LG-30 30º angled Hatching  –  30  10-12µm  – 0.5mm
MAH-LG-35 35º angled Hatching  –  35  10-12µm  – 0.5mm
MAH-LG-45 45º angled Hatching  –  45  10-12µm  – 0.5mm

TPC Assisted Hatching Micropipettes

Reference Pipette Type Finish Style Tip Angle Internal Diameter (microns) Outer Diameter (microns) Distance tip to bend (mm)
LDS Zona Drilling 30 9 µm 1mm

ORIGIO Zona Dissection Micropipettes

Reference Pipette Type Finish Style Tip Angle Internal Diameter (microns) Outer Diameter (microns) Distance tip to bend (mm)
PZD-0 Straight PZD Micropipet  –  0  –  –  0.5mm
PZD-20 PZD Micropipet  –  20  –  –  0.5mm
PZD-30 PZD Micropipet  –  30  –  –  0.5mm
PZD-35 PZD Micropipet  –  35  –  –  0.5mm

TPC Zona Dissection Micropipettes

Reference Pipette Type Finish Style Tip Angle Internal Diameter (microns) Outer Diameter (microns) Distance tip to bend (mm)
LCS Zona Cutting 30 1mm
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