increase in ongoing implantation rate with GM-CSF containing media
Cytokines: a critical role in communications
Cytokines drive the dialogue between the embryo and endometrium and are increasingly expressed throughout embryo development5.
The cytokine GM-CSF is a natural signaling molecule that allows for both autocrine and paracrine communication between the embryo and endometrium.
Providing a more physiological in vitro environment
Our innovative culture media suite is supplemented with the cytokine GM-CSF to mimic the environment found
in the female reproductive tract at conception. Creating the best possible in vitro conditions for the embryo, with the use of this cytokine, will promote successful implantation through improved endometrial receptivity.
For IVF or ICSI, media that supports both gametes during fertilization and promotes sperm function is recommended.
Following fertilization, the embryo immediately begins to communicate with its environment and should be moved to media that better supports the next stage
of its development.
Cytokines are critical in the communication between the embryo and the endometrium prior to implantation and are naturally found in the reproductive tract at this stage.
In the laboratory setting, exposure of embryos to GM-CSF has been shown to promote blastocyst formation and alleviate the negative effects of in vitro culture.
Apposition, adhesion & trophoblast invasion
Maternal-embryo communication is essential for recognition and implantation of the embryo.
Continuing to culture in media containing GM-CSF supports the embryo’s development right through to the blastocyst stage.
From embryo transfer onwards
An environment that supports the dialogue between the embryo and endometrium is crucial for successful implantation, especially for poor prognosis patients. GM-CSF is known to play an important role in this dialogue, as well as the regulation of the maternal immune response.
Transferring embryos in media with GM-CSF ensures that the cytokine is present in the reproductive tract at this vital time.
GM-CSF culture media in the clinical setting
The introduction of a GM-CSF containing culture medium to the IVF world was based on the positive results of EmbryoGen in a prospective randomized clinical trial2, which showed its positive effect on ongoing implantation and live birth rates.
3 days of embryo culture in GM-CSF containing medium improved live birth rate2
Subgroup analysis (n=289 previous miscarriage patients) from a multi-center, randomized, controlled parallel group, double blinded trial with 1,300+ patients from 14 centers.
Data on the clinical use of the full GM-CSF media suite, EmbryoGen and BlastGen, demonstrates that culturing in GM-CSF containing media until the blastocyst stage increases pregnancy and implantation rates6.
EmbryoGen and BlastGen have a positive effect on pregnancy rate and increase the chances of obtaining a live birth6
Blastocyst transfer (frozen-thawed) success rates from a prospective randomized clinical trial with 90 Single Embryo Transfer (SET) cycles using sibling oocytes.
Pregnancy rate: Women with positive hCG at week 2 per women with transfer. Implantation rate: Number of sacs at week 7 per transferred embryo. Ongoing implantation rate: Number of sacs with heart beat at week 7 per transferred embryo. Live birth rate:
Live births per transferred embryo.