Why would you use a spiked injection pipette over a non-spiked pipette?
It is a matter of personal preference. Some embryologists feel that because the non-spiked has a longer bevel it could be damaging, so choose the spiked injection micropipette because it has a shorter bevel with a spike to help it penetrate the wall of the egg.
How does the SlimLine (MIC-SLM) differ from the standard ICSI needle?
The largest difference is in the taper design. The SlimLine taper is more parallel than the standard taper, which is somewhat conical. The length of the taper in the two types is the same.
The Slimline also has a small inner diameter of 4.3-4.9 µm, which is below the diameter of a standard ICSI pipette of 5-5.5 µm.
This design is chosen for minimally invasive ICSI procedures.
I am finding it difficult to control the flow in the ICSI pipette, do you have any suggestions on how to improve that?
The flow in the micropipette is most gentle and subtle in a long, fine tapered pipette. The longest option available in our range is from TPC.
Which is the best bend angle to use?
It doesn’t matter as long as it is aligned with the toolholder. A lower bend angle will restrict the accessible area in high-sided Petri dishes.