SupraSpermTM

Designed for safe and low risk sperm separation and purification by the density gradient method

SupraSperm is an HEPES and Bicarbonate buffered medium containing silane-coated, colloid silica particles.

It is designed to enables motile sperm to be separated from extraneous cells and seminal plasma and to select a subpopulation of cells with the highest capacity for fertilising an oocyte.

Contains SSR® with insulin that is shown to be a pro-survival factor for sperm1, 2.

Key Features

  • Suspension of colloidal silica particles coated with silane
  • With Human Serum Albumin: acts as an anti-sticking effect, antioxidant , scavenger of excess ions and reservoir for cholesterols, fatty acids and vitamins
  • For convenience available both as a ready-to-use gradient system (55% and 80%) and a 100% solution for individual preparation of gradients.
  • Contains SSR® with insulin

Availability

This product is only available for sale in Russia, Australia and Kazakhstan.

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Order Codes

Reference Description Size Shelf Life Guarantee*
10970060 SupraSperm™ 100 100% solution for custom gradient preparation 60 ml Minimum 52 weeks
10972060 SupraSperm™ 100 100% solution for custom gradient preparation 2×60 ml Minimum 52 weeks
10970500 SupraSperm™ 100 100% solution for custom gradient preparation 500 ml Minimum 52 weeks
10922010 SupraSperm™ System 55% + 80% gradients 2×10 ml Minimum 8 weeks
10922060 SupraSperm™ System 55% + 80% gradients 2×60 ml Minimum 8 weeks

*Minimum time to expiry (from date of shipment from Denmark)

  • Calcium Chloride
  • EDTA
  • Glucose
  • HEPES Free Acid
  • HEPES sodium salt
  • Human Albumin Solution (not in SupraSperm 100%)
  • Milli RX Water
  • Magnesium Sulfate
  • Potassium Chloride
  • Potassium Phosphate, monobasic
  • Sodium Chloride
  • Sodium lactate
  • Sodium Pyruvate
  • 1092 only: Gentamicin sulphate
  • Phenol Red
  • Sodium phosphate
  • SSR® (Synthetic Serum Replacement)
  • Sterility tested (Ph. Eur., USP)
  • Osmolality tested (Ph. Eur., USP)
  • pH tested (Ph. Eur., USP)
  • Endotoxin tested ≤ 0.1 EU/ml (Ph. Eur., USP)
  • Sperm survival test
  • 1. Curry, B. J. et al., (2014) Spermatozoa secrete insulin and it has recently been shown that insulin plays a role in capacitation of mammalian spermatozoa, enhancing motility and the acrosome reaction.” Androfert. P-104.
  • 2. Pujianto D.A. et al., (2010). Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation. Endocrinology. 151.